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Image Search Results
Journal: RSC Advances
Article Title: A sequential liquid dispensing method in a centrifugal microfluidic device operating at a constant rotational speed for the multiplexed genetic detection of foodborne pathogens
doi: 10.1039/d4ra04055d
Figure Lengend Snippet: Colorimetric LAMP assays for the detection of foodborne pathogens for four different samples in the centrifugal microfluidic devices. (a) Photograph showing the multiplexed colorimetric LAMP detection of Salmonella spp., V. parahaemolyticus , Campylobacter spp., and norovirus GII after running the assay at 60 °C for 60 min. (b) DNA amplification curves representing the hue changes in the microchambers. (c) Photograph showing the quantitative LAMP detection of Salmonella with DNA concentrations ranging from 10 fg μL −1 to 10 pg μL −1 , where red arrows indicated that the Salmonella -specific LAMP primer set was pre-spotted in the chambers beforehand. (d) The standard curve of Salmonella DNA template, with eight experiment replicates for each DNA concentration.
Article Snippet: Commercially available diagnostic kits containing primer sets and positive control templates for detecting Campylobacter spp. and
Techniques: DNA Amplification, Concentration Assay